METTL3 restricts RIPK1-dependent cell death via the ATF3-cFLIP axis in the intestinal epithelium

Intestinal epithelial cells (IECs) are pivotal for maintaining intestinal homeostasis through self-renewal, proliferation, differentiation, and regulated cell death. While apoptosis and necroptosis are recognized as distinct pathways, their intricate interplay remains elusive. In this study, we report that Mettl3-mediated m6A modification maintains intestinal homeostasis by impeding epithelial cell death. Mettl3 knockout induces both apoptosis and necroptosis in IECs. Targeting different modes of cell death with specific inhibitors unveils that RIPK1 kinase activity is critical for the cell death triggered by Mettl3 knockout. Mechanistically, this occurs via the m6A-mediated transcriptional regulation of Atf3, a transcription factor that directly binds to Cflar, the gene encoding the anti-cell death protein cFLIP. cFLIP inhibits RIPK1 activity, thereby suppressing downstream apoptotic and necroptotic signaling. Together, these findings delineate the essential role of the METTL3-ATF3-cFLIP axis in homeostatic regulation of the intestinal epithelium by blocking RIPK1 activity. Supplementary Information The online version contains supplementary material available at 10.1186/s13619-024-00197-8.


Background
The small intestine epithelium comprises a monolayer of cells organized into distinct structures known as crypts and villi.At the crypt, intestinal stem cells (ISCs) undergo rapid renewal and differentiation, replenishing the stem cell pool and giving rise to differentiated cells (Barker 2014).Homeostasis in the intestinal epithelium relies on continuous renewal and regulated cell death (Okumura and Takeda 2017;Wang et al. 2021).Aberrant increase in cell death is a major contributor to structural disruption in the epithelium, leading to pathological conditions such as inflammatory bowel diseases (IBD) and infectious colitis (Chan 2012;Wang et al. 2022).
It has been reported that intestinal epithelial cells can undergo different types of cell death, including anoikis, apoptosis, necroptosis, ferroptosis and pyroptosis (Patankar and Becker 2020;Subramanian et al. 2020).Receptor-interacting protein kinase (RIPK) 1 is a pivotal protein kinase that mediates both apoptosis and necroptosis (Christofferson et al. 2014;Xu et al. 2021).Upon activation, RIPK1 interacts with FADD, TRADD, and caspase 8 to form the complex II a, promoting RIPK1dependent apoptosis (Micheau and Tschopp 2003).In the presence of caspase inhibitors, activated RIPK1 binds to RIPK3 to form the complex II b, leading to the activation of Mixed-lineage kinase domain-like protein (MLKL) and subsequent necroptosis (Yuan et al. 2019).In the small intestinal epithelium, inhibition of RIPK1 kinase activity suppresses intestinal epithelial cell death and prevents Paneth cell loss in the mice with combined RelA, c-Rel, and RelB deficiency in IECs or IEC-specific knockout (KO) of NEMO (Vlantis et al. 2016).Conversely, specific deficiency of Ripk1 in IECs leads to extensive apoptosis and necroptosis through its scaffold function but not kinase function (Dannappel et al. 2014;Takahashi et al. 2014).Although post-translational modification regulation of RIPK1 has been elucidated in the small intestinal epithelium (Chan 2014), the role of mRNA modifications in intestinal cell death is poorly understood.
N6-adenomethylation (m 6 A) is the most prevalent post-transcriptional modification of eukaryotic RNAs, constituting 0.4%~0.6% of all adenosine nucleotides in mammalian RNAs (Frye et al. 2018).m 6 A methylation is facilitated by a multi-subunit m 6 A methyltransferase complex, composed of methyltransferase-like 3 (METTL3) and 14 (METTL14) (Liu et al. 2014).Removal of m 6 A methylation is orchestrated by RNA demethylases, including FTO (Fat mass and obesity-associated protein) and ALKBH5 (AlkB homolog 5) (Jia et al. 2011;Zheng et al. 2013).Various "reader" proteins mediate the execution of m 6 A functionality, such as RNA stability, splicing, nuclear export, or translation (Zaccara et al. 2019;Zhao et al. 2017).Emerging evidence underscores the indispensability of m 6 A modification in embryonic development, organ growth, and stem cell maintenance (Geula et al. 2015;Li et al. 2018;Liu et al. 2023).m 6 A function in intestine is also appreciated, including ISCs maintenance, epithelial homeostasis and regeneration (Du et al. 2022;Han et al. 2020;Liu et al. 2023;Zhang et al. 2022).However, the role of Mettl3-mediated m 6 A in regulating cell death of the small intestinal epithelium remains elusive.
Here, we report that Mettl3-mediated m 6 A modification is involved in both apoptosis and necroptosis in the small intestinal epithelium.Using mouse and organoid models, we demonstrate that the METTL3-ATF3-cFLIP-RIPK1 axis balances cell survival and death.Mechanistically, ATF3, acting downstream of Mettl3, stimulates the expression of the anti-cell death protein cFLIP, leading to inhibition of RIPK1 and thus cell survival.

Mettl3 deletion induces death of intestinal epithelial cells
In our previous study, we unveiled that METTL3 plays a crucial role in maintaining ISCs in the intestinal epithelium.IEC-specific deletion of Mettl3 compromises stemness without affecting proliferation (Liu et al. 2023).Among the Mettl3-mediated m 6 A modified mRNAs, four encoded transcriptional factors that regulated stemness gene expression in ISCs.Their ectopic expression restored stemness gene expression but could not rescue cell death (Liu et al. 2023).These findings suggest that, beyond its role in stemness regulation, Mettl3-mediated m 6 A methylation is also critical in cell death regulation.Indeed, gene ontology (GO) analysis revealed the genes involved in programmed cell death pathways were enriched among the up-regulated genes in Mettl3-KO IECs (Fig. S1A).
Various programmed cell death mechanisms have been identified in IECs.In addition to apoptosis, necroptosis, pyroptosis, and ferroptosis have been reported in the intestinal epithelium (Patankar and Becker 2020).We first examined the mode of cell death occurring in the Mettl3-KO intestinal epithelium.The hallmarks of apoptosis and necroptosis, cleaved caspase3 (c-caspase 3) and phosphorylated MLKL (p-MLKL), were observed in Mettl3-KO intestinal epithelium at day 4 and day 6 (Fig. S1B, C).Notably, both necroptotic and apoptotic cells were enriched in the crypt region, possibly due to the enriched expression pattern of METTL3 in the crypts (Liu et al. 2023).Based on the cell location and LYZ costaining, a substantial number of necroptotic and apoptotic cells were found in both stem cells and transient amplifying (TA) cells but not Paneth cells (Fig. 1A, B and Fig. S1D, E).Consistently, the transcriptional levels of genes associated with necroptosis and apoptosis were upregulated (Fig. 1C, D), which is confirmed by qPCR analysis (Fig. 1E).Immunoblotting further validated the activation of apoptotic and necroptotic pathways (cas-pase3, RIP3 and MLKL activation) in crypts and organoids derived from Mettl3-KO mice (Fig. 1F and Fig. S1F).Transmission electron microscopy also revealed subcellular features of cell death, including apoptotic body, necrosis-like swelling mitochondria and a low electronic condensation of the cytoplasm in Mettl3-KO crypt cells (Fig. 1G).Additionally, apoptotic and necroptotic signals were detected in the same epithelial cell as shown by p-MLKL and c-caspase3 co-staining (Fig. 1H).Further examination of genes related to apoptosis and necroptosis, such as Casp8 and Ripk1 (Christofferson et al. 2014;Dannappel et al. 2014;Guo et al. 2016), revealed reduced m 6 A modification and elevated gene expression upon Mettl3-KO (Fig. S1G).Together, these observations indicate that Mettl3 depletion triggered extensive apoptosis and necroptosis of intestinal epithelial cells.

Mettl3 regulates ATF3 mRNA stability
Given that METTL3 regulates apoptosis and necroptosis mainly through a RIPK1-dependent manner in IECs, we explored the mechanism how METTL3 regulates RIPK1-dependent cell death.To search for candidates regulated by Mettl3-mediated m 6 A modification, we integrated the RNA-seq, MeRIP-seq and single-cell RNA-seq (scRNA-seq) data for comprehensive analysis.As transcription regulation is mainly involved in altered m 6 A-methylated transcripts (Liu et al. 2023), we predicted upstream transcriptional factors by analyzing the differentially expressed genes associated with cell death using the chEA3 online tool (Fig. S3A).By combining the MeRIP-seq data, RNA-seq data and qPCR confirmation, we found that 4 TFs, Atf3, Csrnp1, Fosb (Liu et al. 2023) and c-Jun, exhibited reduced m 6 A methylation and low mRNA expression in Mettl3 KO cells (Fig. 3A-C and Fig. S3B).Csrnp1 transcript exhibited low expression in the intestinal epithelium (Fig. S3C), and Fosb mainly regulated stemness but not cell death (Liu et al. 2023).
Therefore, we focused on Atf3 and c-Jun, and conducted knockout or overexpression assay to explore their function in organoids (Fig. S3D-F).Organoids subjected to in vitro irradiation conditions undergo organoid damage and cell apoptosis (Kim et al. 2022;Morral et al. 2024).Although Atf3-KO and c-Jun-KO organoids displayed normal morphology, upon irradiation, the survival rate and budding number of these organoids decreased, suggesting that Atf3 and c-Jun play a role in suppressing cell death (Fig. 3D).Conversely, Atf3-overexpression partially restored the survival rate and spheroid size in Mettl3-KO organoids (Fig. 3E).Moreover, Atf3-overexpressing organoids showed reduced levels of cleaved-caspase3, p-MLKL, p-RIPK1 and p-RIPK3 compared to Mettl3-KO organoids (Fig. 3F and Fig. S3G), indicating that Atf3 could inhibit RIPK1-dependent apoptosis and necroptosis.Meanwhile, the total protein level of RIPK3 and MLKL were slightly upregulated upon ATF3 overexpressed, consistent with previous study that ATF3 could regulate the expression of Ripk3 and Mlkl (Inaba et al. 2023).In contrast, the organoids that overexpressed c-Jun exhibited the opposite effect (Fig. 3E).This may attribute to the dual function of c-Jun in both cell survival and cell death (Eferl and Wagner 2003).

ATF3 upregulates cFLIP to suppress cell death
To further investigate the mechanism by which ATF3 regulates RIPK1-dependent cell death signaling in the intestinal epithelium, we performed ChIP-seq to identify ATF3 downstream targets.ATF3 binding was enriched in the DNA motif GG/AGG CGG G/A (Fig. S4A), and its target genes were implicated in the processes of apoptotic process and DNA damage (Fig. 4A).As ATF3 weakly bound to the Ripk1 gene loci (Fig. S4B), by combining Atf3 targets and Mettl3-KO-induced differentially expressed cell death genes, we identified Cflar (Fig. S4C), which encoded cFLIP (Cellular FLICE-like inhibitory protein), a suppressor of RIPK1-mediated cell death complex (Davidovich et al. 2023;Delanghe et al. 2020;Silke and Strasser 2013).Indeed, ATF3 bound to the Cflar gene, and the loci were correlated with the accessible chromatin, as indicated by assay for transposase-accessible chromatin with sequencing (ATAC-seq) analysis (Fig. 4B, C).The expression of Cflar was upregulated in Atf3-OE organoids and downregulated in Mettl3-KO ISCs and crypt cells (Fig. 4D and Fig. S4D, E), implying the Cflar was positively regulated by ATF3.More importantly, compared to wild-type organoids, Cflar-KO organoids exhibited a lower survival rate, fewer budding number and elevated c-caspase3 (Fig. 4E and Fig. S4F).Furthermore, Cflar-overexpression partially rescued the survival rate and spheroid size of Mettl3-KO organoids (Fig. 4F).Co-immunoprecipitation assay revealed cFLIP interacted with RIPK1 (Fig. 4G).Together, these data indicate that cFLIP, as a target of ATF3, interacts with RIPK1 and participates in Mettl3 KO-induced cell death in the intestinal epithelium.

Discussion
It has been reported that m 6 A modification plays an important role in regulation of stemness of ISCs and regeneration of the intestinal epithelium (Han et al. 2020;Liu et al. 2023).In this study, we found that METTL3mediated m 6 A modification is crucial for regulating cell death of the small intestinal epithelial cells, thereby ensuring the maintenance of intestinal epithelial homeostasis (Fig. 6), which is in accordance with the role of m 6 A modification in preserving colon epithelial cell survival by targeting apoptosis (Du et al. 2022;Zhang et al. 2022).Interestingly, we observed that Mettl3-deficiency could induce both apoptosis and necroptosis in small intestinal epithelial cells.Moreover, we found that both apoptosis and necroptosis markers were in a minimal fraction of the same intestinal epithelial cells, indicating that these cells may even undergo concurrent apoptosis and necroptosis.It is worthy of further investigation as to how apoptosis and necroptosis take place in single cell.Inhibition of RIPK1 could prevent Mettl3-KO-induced cell death, whereas the pan-caspase inhibitor or RIPK3 inhibitor did not, demonstrating the essential role of RIPK1 in Mettl3-KO induced cell death.That is consistent with the previous report that RIPK1 could mediate apoptosis and necroptosis via TNFR1 signaling cascades (Xu et al. 2021).
IECs have been reported to undergo different types of cell death (Patankar and Becker 2020;Subramanian et al. 2020).TAK1 and caspase 8 are identified as pivotal regulators of IEC apoptosis versus necroptosis programs downstream of TNFR1 (Fritsch et al. 2019;Geng et al. 2017;Newton et al. 2019).TAK1-activated NF-κB signaling is required for cell survival (Kajino-Sakamoto et al. 2008;Nenci et al. 2007;Pasparakis 2009;Steinbrecher et al. 2008;Sträter and Möller 2000).Their deletion triggers the apoptotic cascade through the recruitment of FADD and caspase 8 to TNF receptor complex (Patankar and Becker 2020).Ablation of FADD or caspase 8 in IECs leads to non-apoptotic cell death and spontaneous gut inflammation, which can be reversed by further knockout of RIPK3 (Günther et al. 2011;Welz et al. 2011).These results indicate that FADD or caspase 8 deficiency causes intestinal inflammation by sensitizing IECs to RIPK3-dependent necroptosis.TAK1 has also been shown to enhance FLIP degradation, facilitating the formation of the RIPK1-FADD-caspase 8 complex, leading to apoptosis (Guo et al. 2016).Moreover, an optimal level of caspase 8 activity is essential for maintaining IEC stability: high activation initiates apoptosis while low activity leads to necroptosis (Ruder et al. 2018).Besides METTL3-ATF3-cFLIP-RIPK1 axis, we also observed that Mettl3 deletion induces an increased expression of Ripk1 and Casp8 (Fig S1 F, G).The upregulation of total Casp8 Fig. 6 A Schematic overview depicting the role of METTL3 in regulating apoptosis and necroptosis in the intestinal epithelium.In intestinal epithelial cells, ATF3 is regulated by METTL3-mediated m 6 A modification.ATF3 binds to and regulates the expression of Cflar, the gene encoding the anti-cell death protein cFLIP, which inhibits RIPK1-mediated apoptosis and necroptosis.Mettl3 deficiency leads to its low expression, high RIPK1 activity and consequently cell death and Ripk1 expression resulting from Mettl3 knockout may induce increased apoptosis and partially inhibit necroptosis.However, necroptosis still occurs in the intestinal epithelial cells by combining the effects of the METTL3-ATF3-cFLIP-RIPK1 axis.Moreover, METTL3 could regulate Ripk1 gene expression through m 6 A modifications, as well as inhibit RIPK1 activation via the METTL3-ATF3-cFLIP-RIPK1 axis.
We identified two transcriptional factors regulated by METTL3-mediated m 6 A modification, Atf3 and c-Jun, which belong to the members of the activator protein-1 (AP-1) family.AP-1 transcription factors regulate cell proliferation, cell survival and cell death (Shaulian and Karin 2002).ATF3 was shown to maintain intestinal barrier regeneration and IEC survival (Yang et al. 2009;Zhou et al. 2017), while it also promotes IEC apoptosis by working with p53 in Crohn's disease (Gu et al. 2018).
Here, we demonstrated that Mettl3-mediated m 6 A modification is essential for Atf3 mRNA stability.We further showed that ATF3 prevents apoptosis and necroptosis by upregulating cFLIP, a suppressor of RIPK1.Therefore, ATF3 plays a crucial role in safeguarding intestinal barrier under stress.c-Jun knockout also enhanced cell death in intestinal organoids, supporting its role in mediating Mettl3-KO-induced cell death.However, c-Jun overexpression also triggered cell death in organoids, which is consistent with the early report (Bossy-Wetzel et al. 1997).These data emphasize the context-dependent role of c-Jun in cell proliferation and death (Shaulian andKarin 2001, 2002), which need further investigation.

Mice
Mettl3 fl/fl mice were from Dr. Wei Li (Institute of Zoology, CAS) (Xu et al. 2017), and Vil-CreERT2 mice were a gift from Dr. Sylvie Robine (Institut Curie-CNRS) (Ireland et al. 2004).Ripk1 K45A mice from Dr. Xiao-Dong Wang (National Institute of Biological Sciences) (Liu et al. 2017).Above mice were crossed to generate Vil-CreERT2;Mettl3 fl/fl mice and Vil-CreERT2;Mettl3 fl/ fl ;Ripk1 K45A mice.Male mice aged 2~4 months were used in this study.All animal studies were conducted in accordance with the relevant guidelines and under the approval of the Institutional Animal Care and Use Committee of Tsinghua University.
For Cre induction in mice intestinal epithelium, Vil-CreERT2;Mettl3 fl/fl mice and Vil-CreERT2;Mettl3 fl/ fl ;Ripk1 K45A mice were intraperitoneally injected at a dose of 100 mg/kg body mass tamoxifen (Sigma, T5649) in corn oil for 4 consecutive days.Mettl3 fl/fl mice and Met-tl3 fl/fl ;Ripk1 K45A mice were treated with an equal amount of tamoxifen as corresponding control group.

Isolation of intestinal crypts and organoid culture
Mouse small intestinal crypts were isolated and cultured following the established procedures (Qi et al. 2017).Briefly, the mouse intestine was longitudinally cut and washed with cold PBS.After carefully removing villi, small intestine pieces were incubated in PBS containing 2 mM EDTA for 30 min on ice.The pieces were then vigorously suspended in cold PBS, and the mixture was filtered through a 70 μm cell strainer (BD Biosciences).The crypt fraction was enriched by centrifugation.Then the crypts were embedded in Matrigel (Corning, #356,231) and seeded on 24-well plate.After polymerization, the crypts were cultured in ENR medium (Advanced DMEM/F12 containing EGF (50 ng /mL, Invitrogen), Noggin (100 ng /mL, OrganRegen) and R-spondin1 (500 ng /mL, Organ-Regen), Penicillin/Streptomycin, GlutaMAX-I, N2, B27 and N-acetylcysteine (Sigma-Aldrich).For passaging, the organoids were suspended with cold PBS, centrifuged and then re-embedded in fresh Matrigel before seeding on a plate.
Retrovirus, lentivirus and recombinant adeno-associated virus (AAV) were produced as previously described (Koo et al. 2011;Li et al. 2021;Liu et al. 2021).Before virus infection, organoids underwent a two-day culture with expansion medium supplemented with 10 mM nicotinamide.Subsequently, the organoids were digested to cell pellet using TrypLE (Gibco,12,604,021).The cell pellet was re-suspended in expansion medium supplemented with 10 µg/mL polybrene (Macgene, MC032), and mixed with the virus.The cell-virus mixture (250 µL) in expansion medium plus polybrene was then added to pre-solidified Matrigel and incubated overnight at 37 °C.Next day, the medium was removed, and the virus was washed with warm PBS.Following this, a 10 µL Matrigel overlay was applied, and the organoids were cultured in expansion medium.
Vil-CreERT2;Mettl3 fl/fl organoids were prepared for retrovirus and lentivirus infection, and the culture medium was replaced with ENR supplemented with 2 µg/mL puromycin at day 2 post-infection.Once the organoids exhibited normal growth under puromycin selection, both protein expression and Mettl3-KO was induced using 4-OHT (0.5µM, Sigma, H7904).Vil-CreERT2;Rosa26 loxp−stop−loxp−Cas9−EGFP organoids were prepared for recombinant AAV infection and pretreated with 4-OHT to induce Cas9 expression.The sgRNA sequences are listed in Supplementary Table S1.

Tyramide signal amplification (TSA)-mediated immunofluorescence and H&E staining
A fluorescence kit based on TSA technology was employed following the manufacturer's instruction (Recordbio Biological Technology, RC0086-23R).Intestinal tissues were fixed in 10% formalin at room temperature for 2 h, dehydrated in 20% sucrose solution overnight, and subsequently embedded for cryo-sections.For antigen retrieval, sections underwent boiling at 100 °C for 10 min in a pH 6.0 citric acid buffer solution within a microwave-enabled retrieval chamber.To inhibit endogenous peroxidase activity, sections were treated with a 3% hydrogen peroxide solution at room temperature for 15 min.3% bovine serum albumin was applied to block non-specific signals for 30 min at room temperature.The sections were then incubated overnight at 4 °C with the primary antibody.Next day, the sections were incubated with horseradish peroxidase-conjugated secondary antibody (anti-mouse or anti-rabbit) for 50 min at room temperature.Following the incubation, fluorescence labeling was achieved by treating the sections with TYR520 fluorescent dye for 10~15 min.For cleaved-caspase 3 (1:300, CST, 9664S) and p-MLKL (1:1600, Abcam, ab196436) co-staining, repeat previous steps from the antigen retrieval, and switch to TYR570 fluorescent dye for p-MLKL.After incubation for fluorescent dye, DAPI was staining for another 15 min.
For hematoxylin-eosin staining, the intestinal tissues were fixed in 4% paraformaldehyde at room temperature overnight, then sequentially in 70%, 80%, 90%, 95%, and 100% ethanol, followed by further dehydration in xylene.The sample was placed in paraffin, immersed for 2 h, and subsequently embedded to create paraffin-sections.Paraffin-sections were automatically stained using a stainer integrated workstation (Leica ST5020-CV5030), the time of hematoxylin staining was 30s.

Transmission electron microscopy (TEM)
The mice intestine was longitudinally opened and cut into approximately 5 mm pieces, followed by fixation in 2.5% glutaraldehyde in PBS immediately.After embedding in Epon Araldite, ultrathin sections were sliced along the crypt-villus axis and analyzed using a Hitachi HT-7800 TEM.

Immunoblotting
Protein lysates were prepared from intestinal epithelial crypts or organoids.Cells were lysed in RIPA buffer (Beyotime, P0013B) with protease inhibitors (Roche, 04693132001), PMSF (Beyotime, ST506) and phosphatase inhibitor (Solarbio, P1260).40 µg of total protein was separated with 10% SDS-PAGE gel under denaturing conditions and were transferred to nitrocellulose membranes (PALL,66,485).Following blocking, the membranes were incubated with primary antibodies and subsequently with secondary anti-rabbit or antimouse conjugated antibodies.Relative protein intensities were calculated by Image Lab software (Version 6.1), the relative level of total proteins was normalized to GAPDH and the relative level of phosphorylated proteins was normalized to the corresponding total protein.

Immunoprecipitation
Co-immunoprecipitation conducted using Dynabeads Protein A/G immunoprecipitation Kit (10007D, Invitrogen) following the manufacturer's instruction.For each sample, 10 µL Dynabeads Protein A and 10 µL Dynabeads Protein G were transferred to a low-binding tube, placed on a magnet, and the supernatant was removed.The beads were then resuspended in 200 Binding & Washing Buffer containing 1 µg of anti-rabbit-IgG (CST, 7074) or rabbit anti-cFLIP (CST, 56,343S) per sample, followed by a 4-hr incubation with rotation at 4 °C.The Dynabeads-antibody complex was separated with a magnet and washed using 200 µL Ab Binding & Washing Buffer.Lentivirus-infected organoids, either control or overexpressing Cflip, were lysed in 300 µL ice-cold IP lysis buffer (10 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.5% NP-40, 1 mM EDTA, 1mM PMSF, 50 mM NaF, 2 mM Na 3 VO 4 , protease inhibitor cocktails (04693132001, Roche)) for 1 h.After centrifugation at 13,000 rpm for 10 min.30 µL soluble supernatants were harvested as input after preclearing, and the remaining soluble supernatants were immunoprecipitated with corresponding antibody binding Dynabeads with rotation overnight at 4 ℃.The immunoprecipitants were washed three times with PBS.The Dynabeads-antibody-antigen complex was gently resuspended in 30 µL loading buffer and analyzed by western blot.

RNA-seq
Total RNA was extracted with RNeasy Mini Kit (QIA-GEN) according to the manufacturer's instruction.Bulk RNA-seq was performed using the Illumina Hiseq X Ten platform and analyzed as previously described (Liu et al. 2023).

ChIP-seq analysis
HISAT2 was used to align the sequences to the mouse genome and generate bam files.PCR duplicates were removed using Picard tools, and Deeptools (3.3.1)bam-Coverage (CPM normalized and extended reads) was applied to generate bigwig files from bam files.MACS2 (v2.2.5) conducted peak calling and generated bed files from aligned reads.HOMER (v4.10.0)annotatePeaks.pl was used for peak annotation.Visualization of the binding peaks from bigwig files was performed using IGV (2.6.2).The motif of ATF3 was identified using findMotif-sGenome.plfrom HOMER.

Methylated RNA immunoprecipitation (MeRIP)-seq, ATAC-seq and Single cell RNA sequencing (scRNA-seq) analysis
A dataset (GSE186917) of control and Mettl3-KO was employed in our research (Liu et al. 2023).We conducted visualizations of m 6 A level and the expression profile of Casp8, Ripk1, Atf3, c-Jun, Csrnp1 and Fosb using the original processing results obtained from MeRIP-seq and scRNA-seq data of control and Mettl3-KO mice (GSE186917).Additionally, the binding peak of Cflip was visualized based on the ATAC-seq data.

Statistics
All experiments were independently repeated with at least three biological replicates.Statistical analysis was performed with Graphpad Prism (version 10).Data shown in graphs represent mean ± SD as indicated in the figure legends.Unpaired two-tailed t-test, one-way ANOVA, and two-way ANOVA analysis was used to compare differences as indicated in the figure legends.

Fig. 1
Fig. 1 Mettl3 deletion results in cell death in the intestinal epithelium.A, B Representative images (left) and quantification (right) of cleaved caspase3 + cell (A) and p-MLKL + cell (B) position in jejunum crypts of control (Mettl3 fl/fl ) and Mettl3-KO (Vil-CreERT2;Mettl3 fl/fl ) mice at the indicated time after TAM injection.The cell boundary was defined by E-cadherin.n = 3 mice/group.C, D Expression heatmap of apoptosis (C) and necroptosis (D) -associated genes in Lgr5 + ISCs of control and Mettl3-KO mice at day 3, 4, 6 post tamoxifen (dpt), based on bulk RNA-seq.E qPCR shows the expression of apoptosis and necroptosis-associated genes in the organoids derived from Vil-CreERT2;Mettl3 fl/fl mice at the indicated time after treated with EtOH or 4-OHT for 2 days.Data were from three independent experiments.F Immunoblotting analyses of apoptosis and necroptosis-associated effector proteins in crypt epithelium isolated from control and Mettl3-KO mice at 6 dpt.GAPDH, loading control.Each lane represents one mice sample.n = 2 mice/group.G Representative electron microscopy images of cell death in jejunum crypts in Mettl3-KO mice at 4-dpt.Red asterisk marks apoptotic bodies of apoptotic cells.Yellow asterisk marks swelling mitochondrion, indicating necroptosis.n = 2 mice/ group.H Immunofluorescence co-staining of cleaved caspase3 and p-MLKL in the jejunum of control and Mettl3-KO mice at 6dpt.n = 3 mice/group.All the data represent mean ± SD.The data were analyzed by Two-way ANOVA (E).*P < 0.05, **P < 0.01 and ***P < 0.001.Scale bars: 50 μm (A, B, H), 1 μm (G).Nuclei were counter-stained with DAPI

Fig. 2
Fig. 2 RIPK1 is required for Mettl3 deficiency-induced cell death.A, B PI-staining was performed in the organoids derived from Vil-CreERT2;Mettl3 fl/ fl mice and treated with EtOH or 4-OHT for 2 days in the presence of DMSO, Nec-1 (5µM), GSK872 (10µM) or z-VAD (10µM) for 4, 5, 6 days, as indicated by representative images (A) and statistical analysis (B) of the relative PI area per organoid (n ≥ 10 organoids).Data were from three independent experiments.C Immunoblotting and quantitative analyses of cell death-associated proteins in Vil-CreERT2;Mettl3 fl/fl derived organoids treated with EtOH or 4-OHT for 2 days in the presence of DMSO or Nec-1 for 5 days.GAPDH, loading control.D Functional enrichment analysis of downregulated genes (Nec-1/DMSO in 4-OHT group) in Vil-CreERT2;Mettl3 fl/fl organoids treated with 4-OHT for 2 days in the presence of DMSO or Nec-1 for 5 days.All the data represent mean ± SD.The data were analyzed by Two-way ANOVA (B).*P < 0.05, **P < 0.01 and ***P < 0.001.Scale bars: 50 μm (A)

Fig. 3
Fig.3ATF3 regulates cell death in the intestinal epithelium.A Venn diagram depicting the overlap of predicted transcription factors that regulate cell death-related genes, genes exhibiting altered m 6 A modification, and differentially expressed genes in Mettl3-KO mice.B Expression of Atf3, Csrnp1, Fosb and c-Jun in FACS-sorted Lgr5 high cells from control and Mettl3-Lgr5-KO mice at 4 dpt, revealed by q-PCR.n = 3 mice per group, each dot represents one mouse.C Integrative Genomics Viewer (IGV) tracks displaying MeRIP-seq reads along the Atf3 and c-Jun genes in Lgr5 high ISCs of control and Mettl3-KO mice.Blue reads were from input libraries and red reads from anti-m 6 A immunoprecipitation libraries.The Y axis represents the CPM (count per million) of genes.The yellow boxes of the tracks depict the positions of m 6 A peaks.D Representative morphology (left), survival rate and budding number (right) of organoids, which were derived from Vil-CreERT2;Rosa26 loxp−stop−loxp−Cas9−EGFP mice at 6 days post 0~4 Gy irradiation, and then infected with AAV to deplete Atf3 or c-Jun.Data were from one of three independent experiments.E Representative morphology (upper), survival number and diameter (bottom) of organoids, which were derived from sorted RFP + infected cells from Vil-CreERT2;Mettl3 fl/fl mice, and were infected with retrovirus expressing Atf3, c-Jun, or both Atf3 and c-Jun.The cells were treated with EtOH or 4-OHT for 2 days and subjected for analysis at day 4.About 10,000 cells were seeded in each well for single cell culture.Data were from three independent experiments.F Immunoblotting and quantitative analyses of cell death-associated proteins in organoids derived from Vil-CreERT2;Mettl3 fl/fl mice, The organoids were treated with EtOH or 4-OHT for 2 days and then infected with control or Atf3-expressed lentivirus.GAPDH, loading control.All the data represent mean ± SD.The data were analyzed by One-way ANOVA (D) and Two-way ANOVA (B, E). *P < 0.05, **P < 0.01 and ***P < 0.001.Scale bars: 100 μm (D, E) (See figure on next page.)

Fig. 4 Fig. 5
Fig. 4 ATF3 regulates cell death in Mettl3-KO organoids via cFLIP.A GO functional enrichment analysis of ATF3 target genes.B ChIP-seq and ATAC-seq tracks of ATF3 binding to Cflar gene loci.C ChIP-qPCR analysis of ATF3 binding at regions near Cflar gene promoters in intestinal organoids.Fold change is shown relative to normal-IgG control.Data were from two independent experiments.D Expression of Atf3, Cflar and Ripk1 in organoids derived from WT mice infected with control lentivirus (control) or Atf3-expressed lentivirus (Atf3-OE).Data were from three independent experiments.E Representative images of morphology (left), survival rate and budding number (right) of organoids derived from Vil-CreERT2;Rosa26 loxp−stop−loxp−Cas9−EGFP mice at 4 days, which were treated with EtOH or 4-OHT for 2 days and then infected with lentivirus to knock out Cflar.Data were from one of three independent experiments.F Representative morphology (left), survival number and diameter (right) of intestinal organoids derived from RFP + infected cells sorted from Vil-CreERT2;Mettl3 fl/fl mice, which were infected with retrovirus expressing Cflar.The cells were treated with EtOH or 4-OHT for 2 days and subjected for analysis at day 4.About 10,000 cells were seeded in each well for single cell culture.Data were from one of three independent experiments.G Immunoblotting analyses of co-immunoprecipitation with IgG or cFLIP antibodies in organoids derived from the crypts of Vil-CreERT2;Mettl3 fl/fl mice, which were infected with cFLIP-expressing lentivirus.GAPDH, loading control.Data were from one of three independent experiments.All the data represent mean ± SD.The data were analyzed by Two-way ANOVA (C, D, E, F). *P < 0.05, **P < 0.01 and ***P < 0.001.Scale bars: 100 μm (E, F) 6-Diamidino-2-Phenylindole MeRIP-seq Methylated RNA immunoprecipitation sequencing